Device
Part:BBa_K4491115
Designed by: Yuen Shan Ho Group: iGEM22_Cambridge (2022-10-12)
MegaT_RFP
The design of MegaT is to design a part that contains RBS, mVenus and Terminator to allow modular JUMP assembly. In this case, to make our system modular, we have added SapI restriction enzyme cut sites on either end of the drop out casette of BBa_J04450 so that users can easily replace the dropout casette with the protein of their choice
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 792
Illegal AgeI site found at 904 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1075
Illegal SapI.rc site found at 5
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Categories
Parameters
None |